Part:BBa_K145014:Design

T7 RNA Polymerase with UmuD derived tag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is made using a two-step PCR. The template was part BBa_I712022 and the primers were:

Step I:

primer 1: CCGCTATTTA GCGATCTTGT TCAGTGTGGC TTTCCTTCAC CGATGAACAC GATTAACATC GC
primer 2: CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG

Step II:

primer 1: ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G
primer 2: CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG

Source

Part composed of the T7 RNA Polymerase and the N-terminal degradation tag, but with no scar (no stop codon)